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cell nuclear antigen  (Proteintech)


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    Structured Review

    Proteintech cell nuclear antigen
    Cell Nuclear Antigen, supplied by Proteintech, used in various techniques. Bioz Stars score: 96/100, based on 1335 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/anti+pcna/PCNA+Antibody/pmc12830253-61-13-17
    Average 96 stars, based on 1335 article reviews
    cell nuclear antigen - by Bioz Stars, 2026-09
    96/100 stars

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    Related Articles

    SDS Page:

    Article Title: Restoration of RBM22 overcomes the transcriptional and epigenetic barriers of cardiomyocyte proliferation for heart regeneration.
    Article Snippet: Protein concentrations were AR TI CL E IN P RE SS determined using the BCA protein assay kit (Thermo Fisher Scientific, 23227). .. Proteins were separated by SDS-PAGE and transferred to nitrocellulose membranes (Cytiva, 10600002), which were blocked with 5% non-fat dry milk for 1 h. Membranes were incubated overnight at 4°C with primary antibodies: anti-RBM22 (Proteintech, 22103-1-AP, 1: 1000), anti-CDK4 (Proteintech,11026-1-AP, 1: 1000), anti-CCNA2 (Proteintech, 18202-1-AP, 1: 1000), antiCCNE1 (Proteintech, 11554-1-AP, 1: 1000), anti-PCNA (Proteintech, 10205-2-AP, 1: 1000), anti-SMARCA4 (Abcam, ab110641, 1: 1000), anti-SMARCC1 (Abcam, ab305037, 1: 1000), anti-SMARCC2 (Abways, AY4215, 1: 1000), anti-Lamin A/C (Thermo Fisher Scientific, PA517113, 1: 1000), anti-Histone H3 (Cell Signaling Technology, #9715S, 1: 1000), anti-β-Actin (Cell Signaling Technology, #3700, 1: 2000), anti-GAPDH (Cell Signaling Technology, #5174, 1: 2000), anti-Flag-tag (Cell Signaling Technology, #2044, 1: 1000), and anti-V5-tag (Cell Signaling Technology, #13202, 1: 1000). ..

    Incubation:

    Article Title: Restoration of RBM22 overcomes the transcriptional and epigenetic barriers of cardiomyocyte proliferation for heart regeneration.
    Article Snippet: Protein concentrations were AR TI CL E IN P RE SS determined using the BCA protein assay kit (Thermo Fisher Scientific, 23227). .. Proteins were separated by SDS-PAGE and transferred to nitrocellulose membranes (Cytiva, 10600002), which were blocked with 5% non-fat dry milk for 1 h. Membranes were incubated overnight at 4°C with primary antibodies: anti-RBM22 (Proteintech, 22103-1-AP, 1: 1000), anti-CDK4 (Proteintech,11026-1-AP, 1: 1000), anti-CCNA2 (Proteintech, 18202-1-AP, 1: 1000), antiCCNE1 (Proteintech, 11554-1-AP, 1: 1000), anti-PCNA (Proteintech, 10205-2-AP, 1: 1000), anti-SMARCA4 (Abcam, ab110641, 1: 1000), anti-SMARCC1 (Abcam, ab305037, 1: 1000), anti-SMARCC2 (Abways, AY4215, 1: 1000), anti-Lamin A/C (Thermo Fisher Scientific, PA517113, 1: 1000), anti-Histone H3 (Cell Signaling Technology, #9715S, 1: 1000), anti-β-Actin (Cell Signaling Technology, #3700, 1: 2000), anti-GAPDH (Cell Signaling Technology, #5174, 1: 2000), anti-Flag-tag (Cell Signaling Technology, #2044, 1: 1000), and anti-V5-tag (Cell Signaling Technology, #13202, 1: 1000). ..

    Article Title: Cold Exposure Alleviates Colitis via Parallel Integration of Colonic Mucosal Regeneration and Ileal Antimicrobial Defense
    Article Snippet: Equal amounts of protein lysates were separated by SDS– PAGE and subsequently transferred onto nitrocellulose membranes (Cytiva, Marlborough, MA, USA). .. The membranes were blocked with 5% fish skin gelatin in TBST for 1 h at room temperature and then incubated with the following primary antibodies for 2 h: anti-Cyclin D1 (60186-1-lg, Proteintech), anti-PCNA (15014-1-AP, Proteintech), anti-Lgr5 (30007-1-AP, Proteintech), anti-Ascl2 (21368-1-AP, Proteintech), and anti-β-actin (TA-09, Zhongshan Goldenbridge-Bio, Beijing, China). .. After three washes with TBST, the membranes were probed with DyLight 800-conjugated goat anti-mouse or anti-rabbit secondary antibodies (Kirkegaard & Perry Laboratories, Gaithersburg, MD, USA) at a 1:10,000 dilution for 1 h. Protein bands were visualized and quantified using an Odyssey Infrared Imaging System and its accompanying Odyssey 2.1 software (LI-COR Biosciences, Lincoln, NE, USA).

    Article Title: Extract of Polygala tenuifolia , Angelica tenuissima , and Dimocarpus longan improve skin wound healing in streptozotocin- induced diabetic mouse
    Article Snippet: .. Rehydrated tissue sections were autoclaved in sodium citrate buffer (pH 6.0) for 10 min. After cooling, sections were washed in PBST (0.1% Triton X-100 in PBS) for 5 min and blocked with 5% BSA in 0.1% PBST for 1 h. For immunofluorescence, tissue sections were incubated with anti-arginase1 (66129-1-Ig, Proteintech), anti-PCNA (ab15497, Abcam), and anti-cytokeratin 17 (sc-393002, Santa Cruz Biotechnology) antibodies overnight at 4 °C , respectively . .. A secondary antibody conjugated to Cy5 (ab6563, ab6564, Abcam) was used.

    Article Title: Cold Exposure Alleviates Colitis via Parallel Integration of Colonic Mucosal Regeneration and Ileal Antimicrobial Defense
    Article Snippet: Equal amounts of protein lysates were separated by SDS–PAGE and subsequently transferred onto nitrocellulose membranes (Cytiva, Marlborough, MA, USA). .. The membranes were blocked with 5% fish skin gelatin in TBST for 1 h at room temperature and then incubated with the following primary antibodies for 2 h: anti-Cyclin D1 (60186-1-lg, Proteintech), anti-PCNA (15014-1-AP, Proteintech), anti-Lgr5 (30007-1-AP, Proteintech), anti-Ascl2 (21368-1-AP, Proteintech), and anti-β-actin (TA-09, Zhongshan Goldenbridge-Bio, Beijing, China). .. After three washes with TBST, the membranes were probed with DyLight 800-conjugated goat anti-mouse or anti-rabbit secondary antibodies (Kirkegaard & Perry Laboratories, Gaithersburg, MD, USA) at a 1:10,000 dilution for 1 h. Protein bands were visualized and quantified using an Odyssey Infrared Imaging System and its accompanying Odyssey 2.1 software (LI-COR Biosciences, Lincoln, NE, USA).

    Immunofluorescence:

    Article Title: Extract of Polygala tenuifolia , Angelica tenuissima , and Dimocarpus longan improve skin wound healing in streptozotocin- induced diabetic mouse
    Article Snippet: .. Rehydrated tissue sections were autoclaved in sodium citrate buffer (pH 6.0) for 10 min. After cooling, sections were washed in PBST (0.1% Triton X-100 in PBS) for 5 min and blocked with 5% BSA in 0.1% PBST for 1 h. For immunofluorescence, tissue sections were incubated with anti-arginase1 (66129-1-Ig, Proteintech), anti-PCNA (ab15497, Abcam), and anti-cytokeratin 17 (sc-393002, Santa Cruz Biotechnology) antibodies overnight at 4 °C , respectively . .. A secondary antibody conjugated to Cy5 (ab6563, ab6564, Abcam) was used.

    Immunohistochemistry:

    Article Title: The PRKN/METTL3/CLDN2 axis promotes colorectal cancer development through epigenetic mechanisms.
    Article Snippet: .. For IHC staining as described [21], slides (4 μm) from clinical CRC tumors and mouse xenografts, which were fixed and embedded in paraffin, were probed with anti-METTL3 (rabbit polyclonal (pAb), 1:1,500, Cat. No. 15073-1-AP, Proteintech, Wuhan, China), anti-CLDN2 (rabbit monoclonal (mAb), 1:600, Cat. No. 18932, Cell Signaling Technology, Danvers, MA, USA), anti-Ki67 (mouse mAb, 1:5,000, Cat. No. 9449, Cell Signaling Technology), anti-PCNA (rabbit pAb, 1:3,500, Cat. No. 10205-2-AP, Proteintech), and anti-MMP9 (mouse mAb, 1:900, Cat. No. ab58803, Abcam, Cambridge, UK) antibodies. .. After protein visualization by 3,3’-diaminobenzidine (DAB) assay (Servicebio, Wuhan, China), slides were subjected to hematoxylin counterstaining.

    Staining:

    Article Title: The PRKN/METTL3/CLDN2 axis promotes colorectal cancer development through epigenetic mechanisms.
    Article Snippet: .. For IHC staining as described [21], slides (4 μm) from clinical CRC tumors and mouse xenografts, which were fixed and embedded in paraffin, were probed with anti-METTL3 (rabbit polyclonal (pAb), 1:1,500, Cat. No. 15073-1-AP, Proteintech, Wuhan, China), anti-CLDN2 (rabbit monoclonal (mAb), 1:600, Cat. No. 18932, Cell Signaling Technology, Danvers, MA, USA), anti-Ki67 (mouse mAb, 1:5,000, Cat. No. 9449, Cell Signaling Technology), anti-PCNA (rabbit pAb, 1:3,500, Cat. No. 10205-2-AP, Proteintech), and anti-MMP9 (mouse mAb, 1:900, Cat. No. ab58803, Abcam, Cambridge, UK) antibodies. .. After protein visualization by 3,3’-diaminobenzidine (DAB) assay (Servicebio, Wuhan, China), slides were subjected to hematoxylin counterstaining.



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